Medium Temperature Alpha-Amylase yog dab tsi?
Medium Temperature Alpha-Amylase yog Bacillus subtilis- muab cov enzyme tsim los rau cov hmoov txhuv nplej siab, ua haujlwm zoo ntawm 60℃thiab 80℃. Feem ntau siv hauv brewing, cawv, thiab starch qab zib industries, nws txo viscosity los ntawm hydrolyzing starch rau hauv dextrins. Nws loj hlob hauv PH 6.0-6.5 thiab yuav tsum muaj calcium tsawg.
Txhua Yam Koj Yuav Tsum Paub
Qhov ntsuas kub cuam tshuam li cas rau amylase?
Kev tshawb fawb ntawm amylase tus yam ntxwv qhia tau hais tias qhov zoo tshaj plaws kub ntawm no enzyme yog 90 degree. Lub enzyme tau ruaj khov rau 1 h ntawm qhov kub ntawm 40-50℃thaum nyob rau ntawm 90 degree, 66% ntawm nws qhov siab tshaj plaws ua haujlwm tau poob.
Dab tsi kub tua amylase?
Qee qhov kub thiab txias yog nyiam dua lwm tus, yog, tab sis nws tsis yog txog thaum koj ntaus qhov kub thiab txias uas koj tau tseem ceeb "tua" cov enzyme. Thiab cov denaturing kub rau beta thiab alpha amylase yog hais txog 160℃F (71 degree) thiab 170℃F (77 degree), feem.
Dab tsi kub yog amylase nquag tshaj plaws ntawm?
Qhov zoo tshaj plaws kub rau tib neeg salivary amylase yog kwv yees li 37℃(𝟗𝟖.𝟔℃𝑭), txuam tib neeg lub cev kub. Txawm li cas los xij, qhov kev pom zoo no txawv ntawm qhov chaw: cog amylase feem ntau ua ntawm 40-60 degree, thaum qee cov kab mob amylase (zoo li cov hauv brewing) tuaj yeem ua haujlwm ntawm 50-70℃lossis siab dua. Thaum tshav kub kub ua rau enzyme denaturation, txo kev ua haujlwm.
Kev khov puas muaj kev cuam tshuam rau amylase?
Amylase yog cov enzyme uas ua rau cov hmoov txhuv nplej siab. Qhov nce hauv qhov kub thiab txias yuav maj mam tawg cov ntawv cog lus thiab ua rau cov enzyme denaturation. Qhov ntsuas kub qis lossis khov yuav ua rau cov haujlwm amylase qeeb, tab sis nws yuav tsis ua rau lub enzyme qhov chaw ua haujlwm.
Cov ntsiab lus tseem ceeb:
Cov yam ntxwv tseem ceeb thiab cov txiaj ntsig
Optimal Kub:60℃txog 80 degree, nrog kev ua haujlwm feem ntau qhia txog 60℃rau, hais tias, Nruab Nrab Kub Kub -Amylase los ntawm Molecular Depot.
Qhov zoo tshaj pH:5.5-8.0, nrog rau ntau yam nyiam ntawm 6.0-6.5.
Kev ruaj ntseg:Nyob ruaj khov ntawm qhov kub thiab txias rau lub sijhawm tsim nyog (piv txwv li, mus txog 80-90℃rau lub sijhawm luv).
Daim ntawv thov:
Brewing/Cawv:Siv tau zoo saccharification.
Starch / Textile:Siv rau hauv desizing thiab txo slurry viscosity.
Cov lus qhia siv
Rau ProFood Medium Temperature Alpha-Amylase (20,000 u / ml), ntau npaum li cas suav nrog 0.3L / 1 tuj cov ntaub ntawv rau brewing thiab 0.6L / 1 tuj rau cov hmoov txhuv nplej siab. Kev khaws cia kom zoo suav nrog qhov chaw qhuav, txias kom deb ntawm tshav ntuj ncaj qha.
Cov nyhuv ntawm qhov kub thiab txias ntawm Amylase Activity
Tsawg kub:Enzyme kev ua haujlwm qeeb vim qis zog kinetic.
Optimum Kub:Kev ua haujlwm siab tshaj plaws tshwm sim nyob ib puag ncig 37℃rau tib neeg, qhov twg enzyme / substrate collisions yog optimized.
Kub kub:Beyond the optimum (generally >55 degree), cov qauv protein denatures (hloov cov duab), rhuav tshem cov chaw nquag thiab nres cov tshuaj tiv thaiv.
ANALYTICAL RESULTS
| Cov khoom kuaj | Method / Ref. | Specification | Kev tshwm sim | Xaus |
|---|---|---|---|---|
| Kev txheeb xyuas | Hauv -house ID / daim ntawv thov | Nruab nrab kub -Amylase | Ua raws | Hla |
| Qhov tshwm sim | Pom kev | Lub teeb xim av rau daj - xim av hmoov | Lub teeb xim av hmoov | Hla |
| Kev ua enzyme | Tus neeg muab khoom -amylase assay | NLT 30,000 U/g | 31,600 U/g | Hla |
| Lub luag haujlwm Unit txhais | Tus neeg muab khoom siv -txhais chav tsev | Qhia xwb | U/g qhia | Hla |
| Solubility | Kev ntsuas dej solubility | Dispersible / soluble hauv dej | Ua raws | Hla |
| pH (1% tov) | pH ntsuas | 5.5 – 7.5 | 6.4 | Hla |
| Poob rau ziab | Gravimetric | Tsawg dua lossis sib npaug li 8.0% | 4.7% | Hla |
| Lead (Pb) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 5.0 mg / kg | 0.24 mg/kg | Hla |
| Arsenic (As) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 3.0 mg / kg | 0.11 mg/kg | Hla |
| Cadmium (Cd) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.05 mg/kg | Hla |
| Mercury (Hg) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.01 mg/kg | Hla |
| Tag nrho cov phaj suav | Phaj suav | Tsawg dua lossis sib npaug li 50,000 CFU / g | 2.2 × 10³ CFU/g | Hla |
| Coliforms | Phaj suav | Tsawg dua lossis sib npaug li 30 CFU / g | < 10 CFU/g | Hla |
| E. coli | Kev kuaj tsis tuaj | Tsis zoo / 25 g | Tsis Pom | Hla |
| Salmonella | Kev kuaj tsis tuaj | Tsis zoo / 25 g | Tsis Pom | Hla |
Txheej txheem ntau lawm
