Cellobiase yog dab tsi?
Cellobiase (tseem hu ua 𝛽-}glucosidase) yog ib qho enzyme, tshwj xeeb yog ib feem ntawm tsev neeg cellulase, uas ua rau kev tawg ntawm cellobiose rau hauv ob lub molecules ntawm qabzib. Nws yog ib qho tseem ceeb hauv cellulose hydrolysis, tiv thaiv kev tawm tswv yim inhibition ntawm lwm cov cellulases (endoglucanase / exoglucanase). Cellobiase yog qhov tseem ceeb rau kev tsim cov roj ntsha biofuels (cellulosic ethanol) thiab hauv kev ua zaub mov, xws li txo iab hauv cov txiv hmab txiv ntoo.
Txhua Yam Koj Yuav Tsum Paub
Cellobiase siv rau dab tsi?
Cellobiase yog ib qho enzyme uas tuaj yeem hydrolyze lub substrate nrog - glucosidic daim ntawv cog lus. Nws tuaj yeem siv rau hauv cawv fermentation. Nws tuaj yeem hydrolyze lub -D-glucosidic daim ntawv cog lus ntawm kev txo qhov kawg, uas cov khoom lag luam yog isomalto-oligosaccharide, qab zib ester, glycopeptide thiab lwm yam.
Puas yog cellobiase hloov piam thaj rau cellulose?
Cellobiase tuaj yeem hydrolyze cellobiose rau hauv qabzib; nws plays lub luag haujlwm tseem ceeb hauv cov txheej txheem ntawm cellulose hydrolysis los ntawm kev txo cov khoom inhibition. Txhawm rau rov siv cov enzyme thiab txhim kho cov txiaj ntsig kev lag luam ntawm cellulosic ethanol, cellobiase tau immobilized siv sodium alginate thiab chitosan ua cov nqa khoom los ntawm txoj kev bubbling.
Puas yog cellobiose muaj kev nyab xeeb rau haus?
Cov ntaub ntawv cov ntaub ntawv nrog rau kev tshawb fawb txog tib neeg kev kam rau siab ua los ntawm tus neeg thov kev pab qhia txog qhov kev zam txim ntawm cellobiose noj yuav tsum yog20 g cellobiose txhua hnub rau ib zaug - siv thiab 15 g ob zaug ib hnub (30 g tag nrho) rau kev noj dua.
Dab tsi yog 4 hom enzymes?
Muaj 6 pawg tseem ceeb ntawm cov enzymes:oxidoreductases, transferases, hydrolases, lyases, isomerases, thiab ligases.
Cov ntsiab lus tseem ceeb:
Lub cev tshwm sim:Feem ntau yog los ntawm daj daj mus rau lub teeb xim av, nrog cov xim sib txawv me ntsis raws li purity, raug txheej txheem, los yog ntxiv stabilizers. Cov qauv purity siab dua yuav ua paler.
Qhov chaw:Nws tuaj yeem muab tau los ntawm ntau yam kab mob, suav nrog cov kab mob fungi (xws li Aspergillus, Trichoderma), kab mob, thiab qee yam nroj tsuag, los ntawm fermentation thiab purification txheej txheem.
Kev ruaj ntseg:Raws li cov hmoov, nws feem ntau muaj kev ruaj ntseg zoo dua piv rau cov kua formulations, feem ntau khaws cov dej num rau ncua sij hawm thaum khaws cia rau hauv txias, qhuav.
Kev Siv Hluav Taws Xob:Siv nyob rau hauv, tab sis tsis txwv rau, cov zaub mov - ua kev lag luam rau flavoring thiab aroma, thiab nyob rau hauv zus tau tej cov bioethanol.
Immobilization:Nws tuaj yeem raug immobilized siv cov neeg ua haujlwm zoo li chitosan -alginate, txhim kho nws txoj kev ruaj ntseg thiab kev ua haujlwm zoo hauv kev lag luam.
Lub luag haujlwm hauv Hydrolysis:Nws rhuav tshem cellobiose (𝛽 -D-glucose bond)) kom tso tawm 𝛽 -D-glucose, ua kom tiav qhov degradation ntawm cellulose rau hauv qabzib.
ANALYTICAL RESULTS
| Cov khoom kuaj | Method / Ref. | Specification | Kev tshwm sim | Xaus |
|---|---|---|---|---|
| Kev txheeb xyuas | Hauv -house ID / daim ntawv thov | Cellobiase ({0}}glucosidase) | Ua raws | Hla |
| Qhov tshwm sim | Pom kev | Dawb rau tawm -dawb hmoov | Tawm- hmoov dawb | Hla |
| Kev ua enzyme | -glucosidase assay / tus txheej txheem xa khoom | NLT 5,000 U/g | 5,200 U/g | Hla |
| Lub luag haujlwm Unit txhais | Tus neeg muab khoom siv -txhais chaw | Qhia xwb | U/g qhia | Hla |
| pH (1% tov) | pH ntsuas | 4.5 – 6.5 | 5.1 | Hla |
| Poob rau ziab | Gravimetric / USP 731 | Tsawg dua lossis sib npaug li 8.0% | 4.3% | Hla |
| Lead (Pb) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 5.0 mg / kg | 0.18 mg/kg | Hla |
| Arsenic (As) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 3.0 mg / kg | 0.09 mg/kg | Hla |
| Cadmium (Cd) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.05 mg/kg | Hla |
| Mercury (Hg) | ICP-MS / ICP-OES | Tsawg dua lossis sib npaug li 0.5 mg / kg | < 0.01 mg/kg | Hla |
| Tag nrho cov phaj suav | Phaj suav | Tsawg dua lossis sib npaug li 50,000 CFU / g | 1.9 × 10³ CFU/g | Hla |
| Coliforms | Phaj suav | Tsawg dua lossis sib npaug li 30 CFU / g | < 10 CFU/g | Hla |
| E. coli | Tsis Muaj Kev Xeem | Tsis zoo / 25g | Tsis Pom | Hla |
| Salmonella | Tsis Muaj Kev Xeem | Tsis zoo / 25g | Tsis Pom | Hla |
Txheej txheem ntau lawm
