Ketose 3-epimerase tau nthuav tawm lub luag haujlwm tseem ceeb hauv tsis yog tsuas yog cyclic monosaccharides bioconversion lub tswv yim, lub npe hu ua Izumoring, tab sis kuj nyob rau hauv kev lag luam lom neeg tsim khoom ntawm d-psicose, ib qho tshiab tsawg-calorie tsis tshua muaj suab thaj dav siv nyob rau hauv cov zaub mov thiab kev kho mob industries. Txij li thaum tsis yog - cov tshuaj tiv thaiv enzymatic tuaj yeem txo qis hauv cov kua qaub, me ntsis acidic pH zoo tshaj yog ib qho teeb meem tseem ceeb rau kev tsim khoom lom neeg ntawm d-psicose.
Txhua Yam Koj Yuav Tsum Paub
Dab tsi yog epimerase enzyme?
D-psicose tuaj yeem tau los ntawm cov piam thaj li cas?
Kev tsim tawm ntawm d-psicose los ntawm d-glucose yuav tsum muaj cov co- qhia thiab kev sib koom ua ke ntawm xylose isomerase thiab d-psicose 3-epimerase.
Dab tsi yog qhov txawv ntawm epimerase thiab isomerase?
Isomerases feem ntau yog ua haujlwm los catalyze isomerization ntawm aldoses thiab ketoses, thaum epimerases pab txhawb kev hloov pauv ntawm stereochemistry hauv qab zib molecules nrog ntau yam chiral carbon atoms.
Yuav ua li cas rau glycogen yog tias koj tsis noj carbs?
Tsis noj txaus Carbohydrates
Tsawg-carb lossis txwv tsis pub noj zaub mov tuaj yeem txo cov khw muag khoom glycogen. Txawm hais tias qee tus neeg zam kev txo qis cov carbohydrates kom zoo, lwm tus ua rau qaug zog sai. Koj lub hlwb thiab cov nqaij ntshiv ntau heev ntawm cov piam thaj. Tsis muaj kev noj zaub mov txaus carbohydrate, glycogen khw muag khoom poob qis.
Cov ntawv thov tseem ceeb:
hauv Kev Lag Luam Khoom Noj
Raws li biocatalyst tseem ceeb rau kev sib txuas D-psicose, nws tshwj xeeb catalyzes epimerization ntawm D-fructose ntawm C3 txoj hauj lwm kom ua tau zoo D-psicose.
Tsawg-calorie sweetener ntau lawm: D-psicose yog ib qho zoo tagnrho sucrose hloov nrog kwv yees li 70% ntawm sucrose qab zib thiab tsuas yog 10% ntawm nws cov calories, tsis muaj kev cuam tshuam loj rau cov ntshav qabzib. Nws yog dav siv hauv dej haus, khoom qab zib, khoom ci thiab lwm yam khoom noj qab zib tsawg - qab zib.
Kev txhim kho khoom noj khoom haus: Nws txhawb nqa cov tshuaj tiv thaiv Maillard txhawm rau txhim kho tsw thiab xim hauv cov khoom ci, txhim kho dej - tuav lub peev xwm thiab kev ntxhib los mos, thiab ua kom pom tseeb ntawm cov kua txiv hmab txiv ntoo.
Kev tsim khoom noj khoom haus muaj nuj nqi: D-psicose muaj cov haujlwm physiological xws li inhibiting rog tsub zuj zuj, tswj cov ntshav qabzib thiab tiv thaiv- cov teebmeem inflammatory, yog li nws yog siv rau hauv cov khoom noj rau cov neeg rog rog, ntshav qab zib thiab hyperlipidemia.
hauv Industrial Biotechnology
Kev hloov pauv biomass: Koom nrog kev kho cov khoom pov tseg xws li pob kws stover thiab nplej bran, tso tawm cov suab thaj fermentable, thiab txhim kho cov txiaj ntsig ntawm bioethanol thiab bio{0}}raws li tshuaj.
Pulp thiab ntawv kev lag luam: Txhim kho lignin extraction thiab bleaching efficiency, txo kev siv tshuaj bleaching agents thiab ib puag ncig muaj kuab paug.
Cov khoom siv biobased synthesis: Catalyzes cov khoom qab zib derivatives siv los ua raw cov ntaub ntawv rau degradable ntim thiab lom polymer cov ntaub ntawv.
hauv Tshuaj thiab Biomedicine
Tsis tshua muaj qab zib synthesis: Ua hauj lwm raws li ib tug tseem ceeb cov cuab yeej enzyme rau kev npaj ntawm D-psicose, D-tagatose thiab lwm yam tsis tshua muaj suab thaj, uas yog ib qho tseem ceeb precursors rau kev tshawb fawb thiab kev loj hlob ntawm anti-inflammatory, antioxidant thiab neuroprotective tshuaj.
Diagnostic reagents: Recombinant DPEase tuaj yeem siv los ua ib qho enzymatic reagent rau kev tsom xam cov qauv carbohydrate thiab pab tshawb nrhiav cov ntsuas glycometabolism.
ANALYTICAL RESULTS
| Cov khoom kuaj | Method/Reference | Cov ntaub ntawv Specification | Cov neeg sawv cev tshwm sim | Xaus |
|---|---|---|---|---|
| Enzyme Lub Npe / Tus Kheej | EC database / ExPASy | D-Psicose 3-epimerase | EC 5.1.3.30; Lwm lub npe: D-allulose 3-epimerase, DPEase (Expasy ENZYME) | Ua raws |
| Catalyzed Reaction | ExPASy enzyme nkag | Reversible C-3 epimerization | D-allulose ⇌ keto-D-fructose (Expasy ENZYME) | Ua raws |
| Substrate Specification | ExPASy / UniProt | Siab tshwj xeeb rau D-psicose / D-fructose khub | Tshwj xeeb heev rau D-psicose; Kev ua haujlwm qis heev nrog D-tagatose (Expasy ENZYME) | Ua raws |
| Cofactor Requirement | Cov ntawv sau ua cim / UniProt | Hlau -dependent enzyme | Mn²⁺ nyiam; Co²⁺ kuj active; Mg²⁺/Fe²⁺/Ni²⁺ qhia kev ua haujlwm qis (Springer) | Ua raws |
| Pom meej Molecular Luj (Monomer) | SDS-PAGE / ntawv nyeem | ~ 30-33 hli | Protein band pom ntawm li 30 kDa; kwv yees loj txog 33 kDa (Springer) | Ua raws |
| Quaternary Structure | Crystal qauv ntaub ntawv | Tetrameric enzyme | Native enzyme qhia ua tetramer; txhua monomer belongs rau TIM- chim quav (RCSB PDB) | Ua raws |
| Qhov zoo tshaj pH | Enzymatic kev ntsuam xyuas | Feem ntau nyob ze nruab nrab | Cov neeg sawv cev zoo tshaj pH=7.5 (qhia rau cov khoom noj sib xyaw ua ke- cov qauv tsim muaj feem) (Springer) | Ua raws |
| Qhov kub thiab txias | Enzymatic kev ntsuam xyuas | Nruab nrab thermal kev ua haujlwm | Tus sawv cev qhov kub zoo tshaj=50–55℃(Springer) | Ua raws |
| Pom zoo Mn²⁺ Qib rau kev hloov pauv | Txheej txheem characterization | Tsawg-mM qib | 1 mM Mn²⁺ qhia rau IfDPEase; 10 mM Mn²⁺ qhia rau recombinant A. tumefaciens DPEase txheej txheem optimization (MDPI) | Ua raws |
| Tus Neeg Sawv Cev Hloov Pauv Kev Ua Haujlwm A | Cov ntaub ntawv txheej txheem cov ntaub ntawv | Cov ntaub ntawv nkaus xwb | Ntawm pH 7.5, 55 degree, 10 mM Mn²⁺: 25% (w/v) fructose muab 5.60% (w/v) D-psicose; Hloov pauv tus nqi 22.42% (Springer) | Cov ntaub ntawv |
| Tus Neeg Sawv Cev Hloov Ua Haujlwm B | Cov ntaub ntawv txheej txheem cov ntaub ntawv | Cov ntaub ntawv nkaus xwb | Ntawm pH 7.5, 50 degree, 1 mM Mn²⁺: 36.1% D-psicose tau los ntawm 10 mg/mL D-fructose (MDPI) | Cov ntaub ntawv |
| Daim ntawv thov cuam tshuam | EFSA / industrial ntaub ntawv | Siv rau D-allulose ntau lawm | Khoom noj khoom haus enzyme npaj ntawm D-psicose 3-epimerase raug tshuaj xyuas rau kev siv fructose- rau-D-allulose isomerisation txheej txheem (efsa.onlinelibrary.wiley.com) | Ua raws |
Txheej txheem ntau lawm
